Oxidized Low-Density Lipoprotein Increases Bone Sialoprotein Expression in Vascular Smooth Muscle Cells Via Runt-Related Transcription Factor 2

Farrokhi, E. and Ghatreh Samani, K. and Hashemzadeh Chaleshtori, M. (2015) Oxidized Low-Density Lipoprotein Increases Bone Sialoprotein Expression in Vascular Smooth Muscle Cells Via Runt-Related Transcription Factor 2. AMERICAN JOURNAL OF THE MEDICAL SCIENCES, 349 (3). pp. 240-243.

Full text not available from this repository.

Abstract

Background: Vascular calcification is a pivotal stage in atherosclerosis. During vascular calcification, vascular smooth muscle cells (VSMCs) synthesize many osteogenic factors such as bone sialoprotein (BSP). Oxidative stress plays a critical role in progression of atherosclerosis and also increases extracellular matrix proteins expression. BSP overexpression has been observed during vascular calcification by oxidative stress. However, the regulatory mechanism of oxidized low-density lipoprotein (oxLDL)-mediated vascular calcification has not yet been fully defined. In this study, we aimed to investigate whether runt-related transcription factor 2 (Runx2) affects the oxLDL-induced BSP expression or not. Methods: In this experimental study, we cultured VSMCs in F12K media and then treated them with oxLDL. The expression of Runx2 and BSP genes was determined by real-time polymerase chain reaction method. Protein level of each gene was investigated by Western blotting technique. To determine whether Runx2 regulates BSP gene expression at VSMCs induced by oxLDL, we suppressed Runx2 mRNA using siRNA. Transfected cells then were treated with oxLDL and expression of Runx2 and BSP genes was determined again. Results: oxLDL increased Runx2 and BSP expression (4.8 +/- 0.47-fold and 4.91 +/- 0.56-fold, respectively) after 48 hours. Western blotting method confirmed the increased levels of Runx2 and BSP proteins after 48 hours. Runx2 overexpression alone induced BSP expression, whereas knockdown of Runx2 with small interfering siRNA blocked oxLDL-induced BSP expression. Conclusions: Our results showed that oxLDL-induced BSP expression was dependent on Runx2 expression, suggesting that Runx2 is required for oxLDL-induced BSP expression.

Item Type: Article
Uncontrolled Keywords: Vascular calcification; Oxidized low-density lipoprotein; Bone sialoprotein; Runx2
Subjects: WE Musculoskeletal system
QU Biochemistry
Divisions: Faculty of Medicine
Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Clinical Biochemistry Research Center
Depositing User: zahra bagheri .
Date Deposited: 22 Jul 2017 07:12
Last Modified: 22 Jul 2017 07:12
URI: http://eprints.skums.ac.ir/id/eprint/1639

Actions (login required)

View Item View Item