Edaravone leads to proteome changes indicative of neuronal cell protection in response to oxidative stress

Jami, Mohammad-Saeid. and Salehi-Najafabadi, Zahra. and Ahmadinejad, Fereshteh. and Hoedt, Esthelle. and Hashemzadeh-Chaleshtori, Morteza. and Ghatreh samani, Mahdi. and Neubert, Thomas.A. and Larsen, Jan Petter. and Moller, Simon Geir. (2015) Edaravone leads to proteome changes indicative of neuronal cell protection in response to oxidative stress. NEUROCHEMISTRY INTERNATIONAL, 90. pp. 134-141.

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Abstract

Neuronal cell death, in neurodegenerative disorders, is mediated through a spectrum of biological processes. Excessive amounts of free radicals, such as reactive oxygen species (ROS), has detrimental effects on neurons leading to cell damage via peroxidation of unsaturated fatty acids in the cell membrane. Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) has been used for neurological recovery in several countries, including Japan and China, and it has been suggested that Edaravone may have cytoprotective effects in neurodegeneration. Edaravone protects nerve cells in the brain by reducing ROS and inhibiting apoptosis. To gain further insight into the cytoprotective effects of Edaravone against oxidative stress condition we have performed comparative two-dimensional gel electrophoresis (2DE)-based proteomic analyses on SH-SY5Y neuroblastoma cells exposed to oxidative stress and in combination with Edaravone. We showed that Edaravone can reverse the cytotoxic effects of H2O2 through its specific mechanism. We observed that oxidative stress changes metabolic pathways and cytoslceletal integrity. Edaravone seems to reverse the H2O2-mediated effects at both the cellular and protein level via induction of Peroxiredoxin-2. (C) 2015 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Parkinson's disease; Edaravone; Peroxiredoxin-2; L-Dopa; Oxidative stress; Proteomics
Subjects: WL Nervous system
QU Biochemistry
Divisions: Reserach Vice-Chancellar Department > Cellular and Molecular Research Center
Reserach Vice-Chancellar Department > Medical Plants Research Center
Depositing User: zahra bagheri .
Date Deposited: 19 Jul 2017 06:11
Last Modified: 04 Mar 2018 06:37
URI: http://eprints.skums.ac.ir/id/eprint/1579

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