Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens

Mokhtarian, Kobra. and Akhlaghi, Lame. and Meamar, Ahmad Reza. and Razmjou, Elham. and Manouchehri Naeini, Kourosh. and Gholami, Samaneh. and Najafi Samei, Masoomeh. and Falak, Reza. (2016) Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens. PARASITOLOGY RESEARCH, 115 (8). pp. 2957-2965.

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Abstract

In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used fast protein liquid chromatography (FPLC)-fractionated extracts of F. hepatica excretory/secretory antigens (E/S Ags) for serodiagnosis of human and sheep fasciolosis. To develop an improved diagnostic method, we fractionated F. hepatica E/S Ags by anion exchange chromatography on a Sepharose CL-6B column and then tested the serodiagnostic values of the fractions. We used sera from F. hepatica-infected human and sheep as positive controls. Sera from patients with hydatidosis and strongyloidiasis were used for cross-reactivity studies. Enzyme-linked immunosorbent assays (ELISA) of the second FPLC peak, containing 20, 25, and 70 kDa proteins, discriminated between F. hepatica-infected and uninfected human and sheep samples. Fractionation of F. hepatica E/S Ags by FPLC is a fast and reproducible way of obtaining antigens useful for serodiagnosis of human and sheep fasciolosis with acceptable sensitivity and specificity.

Item Type: Article
Uncontrolled Keywords: Fasciolosis; Fast protein liquid chromatography; Fractionated excretory/secretory antigens
Subjects: WK Endocrine System
QU Biochemistry
Divisions: Faculty of Medicine > School of Medicine
Depositing User: Users 1 not found.
Date Deposited: 16 Jul 2017 05:01
Last Modified: 18 Feb 2018 06:42
URI: http://eprints.skums.ac.ir/id/eprint/1160

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